Muc5ac mucin expression during rat skin development

Muc5ac Mucin Expression During Rat Skin Development

UNLABELLED Some mucin genes have been detected during human embryonic and fetal organ development; however, little is known about mucin expression in epidermal development, neither in humans nor in other species. The present research was developed to explore Muc5ac skin expression during prenatal and postnatal rat development. Immunohistochemistry (IHC), Western blotting (WB) and RT-PCR were em...

Assessment of Glycoconjugates Terminal Sugars Expression During Rat Skin Morphogenesis

Purpose: This study was conducted in order to determine distributions and changes of glycoconjugates terminal sugars during skin morphogenesis. Materials and Methods: using lectin histochemistry technique, 10% formalin fixed, paraffin embedded rat embryonic sections for days 12, 14, 16 of gestation (N=30) were incubated with different HRP-lectins from Lotus tetragonolobus (LTA), Maclura pomifer...

Induction of Mucin and MUC5AC

Acrolein-induced MUC5ac expression in rat airways.

Acrolein, a low-molecular-weight aldehyde found in photochemical smog and tobacco smoke, can induce mucus hypersecretion, inflammation, and airway hyperreactivity. To determine whether changes in steady-state mucin gene expression (MUC2 and MUC5ac) are associated with histological signs of mucus hypersecretion, rats were exposed to acrolein (3.0 parts/million, 6 h/day, 5 days/wk, 2 wk), and the...

Immunohistochemical study of type III collagen expression during pre and post-natal rat skin morphogenesis

Objective(s):Skin extracellular matrix, which contains type I and type III collagens, is involved in skin development. The aim of this study was to investigate type III collagen distribution pattern as well as its changes during pre and post-natal skin morphogenesis in rats. Materials and Methods: Ventral skins of Wistar rat embryos at different stages from 10 to 20 gestational day (E10-E20) a...